From conducting ground breaking experiments in research laboratories to performing quality
tests of materials at a chemical industry, precise and accurate measurement is vital, and so is
the handling of materials. As a solution, there is one common laboratory tool used to measure
as well as transfer liquid from one container to another. This tool is “pipette”- an instrument to
accurately aspirate and dispense liquid from a nozzle to one or more aliquots. Depending upon
the number of channel dispensing liquid at a time, pipettes are classified as single and multi-
channel. This blog will enlighten you on what is Single Channel Micropipette, its benefits and
uses.

Single Channel Micropipette

Single Channel Micropipette is used to aspirate or release precise volume of liquid into a
container, typically is volumes of microliters. These pipettes allow division of large volume of
water into micro volumes called aliquots. This is widely used to manually or automatically
control the volume of liquid being dispensed. Unlike multi-channel micropipette where more than
1 nozzle dispenses the liquid, micropipette provides targeted dispensation into aliquots.

Benefits of Single Channel Micropipette

• Precise Liquid Transfer: Users can set precise volumes in microliters to handle, aspirate and
  dispense the liquid with accuracy. This ensures consistent results and reduces the human error
  in observations.
• Ergonomic: Easy to handle for long without wearing the hands. Available in adjustable volumes
  settings for various measurements.
• Versatile: Many laboratory applications and diagnostics use single channel micropipette as it
  can handle and give accurate results for wide range.
• Experimentation: More often experimentations involve low throughput of liquid and targeted
  dispensation in small aliquots, which can be handled easily by single channel micropipette.
• Easy Calibration: These can be easily calibrated and hence save replacement cost.
  Manufacturers like RV Instruments provide calibration support.
• Reduces Contamination: The single transfer application reduces risk of cross contamination.
  Applications of Single Channel Micropipette

Given the many benefits- targeted transfer, efficiency, cost-effectiveness and compact design,
single channel pipettes have versatile use case in small as well as industrial laboratories:

1. Liquid Handling:

● To measure and dispense small portions of liquid, like solvents, reagents, media,
cell cultures.
● Useful in mixing, diluting and transferring samples.

2. Molecular Biology and Genetics

● Pipetting small aliquots of reagents for DNA analysis
● Loading DNA, RNA, or protein samples into wells.
● Pouring enzymes and buffers for restriction digestion and ligation.

3. Cell Biology and Microbiology

● Pipetting media, buffers, and reagents for cell cultures
● Transferring small volumes of bacterial or cell suspensions.
● Handling dyes and stains for microscopy or flow cytometry.

4. Biochemistry and Analytical Chemistry

● Adding small amount of substrates, enzymes, and buffers.
● Sample preparation for assays.

5. Pharmaceutical and Biomedical Research

● Precise dispensing of test compounds.
● Preparing serial dilutions and test solutions
● Handling small sample volumes for immunoassays.

6. Environmental Testing

● Pouring reagents and samples for chemical or biological analysis
● Aspirating reagents for nutrient or contaminant analysis.

7. Forensic Science

● Precise sample preparation and amplification.
● Preparing and analyzing evidence samples.

8. Food and Beverage Testing

● Transferring liquid samples for microbial assays.
● Reagent handling for chemical testing.

9. Educational and Training Purposes

● Teaching students the basics of precise pipetting techniques.
● Illustrating principles of liquid handling in workshops and training sessions.

10. Quality Control

● Ensuring accurate measurements during product testing.
● Pipetting standard solutions for calibration and testing.

Their versatility, accuracy, and convenience make single-channel micropipettes indispensable
across these fields. If you want to buy from a range of pipettes, RV Instruments is a prominent
manufacturer and supplier of great quality pipette with calibration support. This give highly
accurate measurements for diverse applications.